IFNA2 antibody

Catalog No. ABIN6252254

The Mouse Monoclonal anti-IFNA2 antibody (Clone N27) (ABIN6252254) specifically detects IFNA2 in WB, ELISA, IF, FACS and BI. The antibody is reactive with Human samples.

Quick Overview for IFNA2 antibody (ABIN6252254)

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IFNA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), BioImaging (BI)

Clone: N27

Product Details anti-IFNA2 Antibody

Purification: Protein G affinity chromatography

Immunogen: Purified recombinant human IFNA2 was used as the immunogen for the Interferon alpha 2 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA: order BSA/azide free format for coating

Blocking: order BSA/azide free format

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

IF: 0.5-1 μg/mL

Optimal dilution of the Interferon alpha 2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for IFNA2

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Alternative Name: Interferon alpha 2

Background: Recognizes a protein of 16-27  kDa, identified as human Interferon alpha 2 (IFNA2). Its epitope maps between amino acids 43-53 of the IFNA2. This mAb is specific for IFNA2 and does not cross-react with IFNA1. The epitopes of clones N27 and N39 mAbs are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFNA2 after viral infections. The IFNA family consists of 24 or more genes or pseudo-genes. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.

Gene ID: 3440

UniProt: P01563

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C