CD45RO antibody

Catalog No. ABIN6252434

The Mouse Monoclonal anti-CD45RO antibody (Clone SPM125) (ABIN6252434) specifically detects CD45RO in FACS, IF and IHC. The antibody is reactive with Human, Chimpanzee and Marmoset samples.

Quick Overview for CD45RO antibody (ABIN6252434)

Target: CD45RO

Reactivity: Human, Chimpanzee, Marmoset

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RO antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: SPM125

Product Details anti-CD45RO Antibody

Purification: Protein G affinity chromatography

Immunogen: Cultured human T-cells from an IL-2-dependent T-cell line (CA1) were used as immunogen for this anti-CD45RO antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

Immunofluorescence: 0.5-1 μg/mL

Immunohistology (FFPE): 0.25-0.5 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Due to variation in protocol and secondary antibody used, the anti-CD45RO antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for CD45RO

Target: CD45RO

Alternative Name: CD45RO

Background: Recognizes a 180-185  kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Gene ID: 5788

UniProt: P08575