MCM7 antibody (AA 195-319)

Catalog No. ABIN6252451

The Mouse Monoclonal anti-MCM7 antibody has been validated for WB, IF, FACS and IHC (p). It is suitable to detect MCM7 in samples from Human.

Quick Overview for MCM7 antibody (AA 195-319) (ABIN6252451)

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM7 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MCM7-1467

Product Details anti-MCM7 Antibody

Binding Specificity: AA 195-319

Purification: Protein G affinity chromatography

Immunogen: A human partial recombinant protein corresponding to amino acids 195-319 was used as the immunogen for the MCM7 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Immunofluorescence: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the MCM7 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for MCM7

Target: MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Alternative Name: MCM7

Background: MCM7 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that is essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumor suppressor protein RB1/RB.

Gene ID: 4176

UniProt: P33993

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA