Keratin Acidic (AE1) antibody

Catalog No. ABIN6252466

The Mouse Monoclonal anti- antibody has been validated for IF, WB and IHC (p). It is suitable to detect in samples from Human and Rat.

Quick Overview for Keratin Acidic (AE1) antibody (ABIN6252466)

Target: Keratin Acidic (AE1)

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ACCK1-1

Product Details

Purification: Protein G affinity chromatography

Immunogen: An amino acid sequence common to acidic/type I keratins was used as the immunogen for the Acidic Cytokeratin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Western blot: 1-2 μg/mL (3)

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Titering of the Acidic Cytokeratin antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. This antibody will detect the following cytokeratins (CK): CK10: 56  kDa, CK14: 50  kDa, CK15: 50  kDa, CK16: 48  kDa, CK19: 40  kDa.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: Acidic Cytokeratin

Background: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]

Gene ID: 9606

UniProt: Q7Z794