Keratin Acidic (AE1) antibody

Catalog No. ABIN6252468

This Mouse Monoclonal antibody specifically detects in IHC, IF, WB and FACS. It exhibits reactivity toward Human, Rat, Chicken, Cow, Mouse, Rabbit, Monkey, Dog and Turtle.

Quick Overview for Keratin Acidic (AE1) antibody (ABIN6252468)

Target: Keratin Acidic (AE1)

Reactivity: Human, Rat, Chicken, Cow, Mouse, Rabbit, Monkey, Dog, Turtle

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS)

Clone: AE1

Product Details

Purification: Protein G purified

Immunogen: Solubilized keratin extract from human stratum corneum

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells

IF: 1-2 μg/mL

WB: 0.5-1 μg/mL

IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Acidic Cytokeratin antibody AE1 to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: Acidic Cytokeratin

Background: Cytokeratins, also called keratins or simply CKs, are a family of fibrous structural proteins and a major structural component in the outer layer of human skin, as well as hair and nails.  There are 20 human epithelial keratins which can be divided into two subfamiles: acidic and basic (more recent human genone sequencing has identified an additional 20 members). The formation of cytokeratin intermediate filaments requires the pairing of at least one acidic and one basic subfamily member. Members of the same keratin subfamily share extended sequence homology while members of different subfamilies show only limited sequence homology.

This antibody is specific for the 56.5  kDa (CK10), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), and 40  kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Many studies have shown the usefulness of CKs as markers in cancer research and tumor diagnosis, as epithelial tumors generally maintain the same CK expression patterns as their counterpart normal tissue. Clone AE1 is commonly used with clone AE3 as a pan cytokeratin antibody cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).

Gene ID: 3858

UniProt: P13645