ENO2/NSE antibody (AA 416-433)

Catalog No. ABIN6252647

The Mouse Monoclonal anti-ENO2/NSE antibody (Clone ENO2-1375) (ABIN6252647) specifically detects ENO2/NSE in WB, IHC, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ENO2/NSE antibody (AA 416-433) (ABIN6252647)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ENO2-1375

Product Details anti-ENO2/NSE Antibody

Binding Specificity: AA 416-433

Purification: Protein G affinity chromatography

Immunogen: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

Isotype: IgG2b

Application Details

Application Notes: Western blot: 0.5-1 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

IF: 1-2 μg/mL

IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: Neuron Specific Enolase

Background: Recognizes a protein of about 50  kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

Gene ID: 2026

UniProt: P09104