GFM1 antibody

Catalog No. ABIN6252740

This anti-GFM1 antibody is a Mouse Monoclonal antibody detecting GFM1 in WB, ELISA, IF and IHC (p). Suitable for Human.

Quick Overview for GFM1 antibody (ABIN6252740)

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AE-1

Product Details anti-GFM1 Antibody

Specificity: Does not react with mouse and rat

Purification: Protein G affinity chromatography

Immunogen: A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: ELISA: order Ab without BSA

Immunofluorescence: 0.5-1 μg/mL

Western blot: 0.25-0.5 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

Optimal dilution of the anti-Mitochondrial antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for GFM1

Target: GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))

Alternative Name: Mitochondrial

Background: This mAb recognizes a 60  kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.