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GFM1 antibody

GFM1 Reactivity: Human WB, IF, IHC (p), FACS Host: Mouse Monoclonal MTC719 unconjugated
Catalog No. ABIN3025203
  • Target See all GFM1 Antibodies
    GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
    Reactivity
    • 23
    • 18
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 28
    • 6
    Mouse
    Clonality
    • 28
    • 6
    Monoclonal
    Conjugate
    • 20
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This GFM1 antibody is un-conjugated
    Application
    • 33
    • 13
    • 13
    • 10
    • 10
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    No Cross-Reactivity
    Insect, Bacteria
    Characteristics
    MAb MTC719 recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
    Purification
    Protein G affinity chromatography
    Immunogen
    The Mitochondrial fraction of HeLa cells was used as the immunogen for this Mitochondrial antibody.
    Clone
    MTC719
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product GFM1 Primary Antibody
  • Application Notes
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Mitochondrial antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,WB: 0.25-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
    Alternative Name
    Mitochondrial (GFM1 Products)
    Synonyms
    COXPD1 antibody, EFG antibody, EFG1 antibody, EFGM antibody, EGF1 antibody, GFM antibody, hEFG1 antibody, AW545374 antibody, D3Wsu133e antibody, Gfm antibody, EF-G antibody, Efg antibody, G elongation factor mitochondrial 1 antibody, G elongation factor, mitochondrial 1 antibody, GFM1 antibody, Gfm1 antibody
    Background
    MAb MTC719 recognizes a 60  kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb MTC719 recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
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