TRIM29 antibody (N-Term)

Catalog No. ABIN6252757

The Mouse Monoclonal anti-TRIM29 antibody has been validated for IF and IHC (p). It is suitable to detect TRIM29 in samples from Human.

Quick Overview for TRIM29 antibody (N-Term) (ABIN6252757)

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRIM29 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TPMP29-1

Product Details anti-TRIM29 Antibody

Binding Specificity: N-Term

Purification: Protein G affinity chromatography

Immunogen: An N-terminal human recombinant protein fragment was used as the immunogen for the TRIM29 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Titering of the TRIM29 antibody may be required for optimal performance.

1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TRIM29

Target: TRIM29 (Tripartite Motif Containing 29 (TRIM29))

Alternative Name: TRIM29

Background: Tripartite motif-containing protein 29 interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA.

Gene ID: 23650

UniProt: Q14134

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus