TAG-72 antibody

Catalog No. ABIN6252881

The Mouse Monoclonal anti-TAG-72 antibody (Clone CC49) (ABIN6252881) specifically detects TAG-72 in IHC, FACS, IF and WB. The antibody is reactive with Human, Rat, Cow and Dog samples.

Quick Overview for TAG-72 antibody (ABIN6252881)

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Reactivity: Human, Rat, Cow, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAG-72 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Clone: CC49

Product Details anti-TAG-72 Antibody

Purification: Protein G affinity chromatography

Immunogen: Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.

Isotype: IgG1

Application Details

Application Notes: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Target Details for TAG-72

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Alternative Name: TAG-72

Background: Recognizes an oncofetal antigen of 220  kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.