RPS6KB1 antibody (pThr449)

Catalog No. ABIN6254043

The Rabbit Polyclonal anti-RPS6KB1 antibody has been validated for WB and ELISA. It is suitable to detect RPS6KB1 in samples from Arabidopsis.

Quick Overview for RPS6KB1 antibody (pThr449) (ABIN6254043)

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivity: Arabidopsis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RPS6KB1 Antibody

Binding Specificity: pThr449

Specificity: Specific for endogenous levels of the ~53 kDa S6K1 protein phosphorylated at Thr449. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Arabidopsis

Characteristics: Our S6 Kinase 1 (Thr449) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects S6 Kinase 1 (Thr449) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr449 of Arabidopsis S6K1, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for RPS6KB1

Target: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Alternative Name: S6K1

Background:

Synonyms: 70 kDa ribosomal protein S6 kinase 1 antibody, KS6B1_HUMAN antibody, p70 alpha antibody, P70 beta 1 antibody, p70 ribosomal S6 kinase alpha antibody, p70 ribosomal S6 kinase beta 1 antibody, p70 S6 kinase alpha antibody, P70 S6 Kinase antibody, p70 S6 kinase alpha 1 antibody, p70 S6 kinase alpha 2 antibody, p70 S6K antibody, p70 S6K-alpha antibody, p70 S6KA antibody, p70(S6K) alpha antibody, p70(S6K)-alpha antibody, p70-alpha antibody, p70-S6K 1 antibody, p70-S6K antibody, P70S6K antibody, P70S6K1 antibody, p70S6Kb antibody, PS6K antibody, Ribosomal protein S6 kinase 70 kDa polypeptide 1 antibody, Ribosomal protein S6 kinase beta 1 antibody, Ribosomal protein S6 kinase beta-1 antibody, Ribosomal protein S6 kinase I antibody, RPS6KB1 antibody, S6K antibody, S6K-beta-1 antibody, S6K1 antibody, Serine/threonine kinase 14 alpha antibody, Serine/threonine-protein kinase 14A antibody, STK14A antibody

Description Logic: Our S6 Kinase 1 (Thr449) rabbit polyclonal phosphospecific primary antibody is produced . It detects S6 Kinase 1 (Thr449) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: S6K1

Molecular Weight: 53 kDa

Gene ID: 820020

UniProt: P42818

Pathways: PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, Warburg Effect