RFP antibody

Catalog No. ABIN6254205

Cited in 4+ publications. The Goat Polyclonal anti-RFP antibody (ABIN6254205) specifically detects RFP in WB, IF, IHC (fro) and IHC (p). The antibody is reactive with Discosoma samples.

Quick Overview for RFP antibody (ABIN6254205)

Target: RFP (Red Fluorescent Protein (RFP))

Reactivity: Discosoma

Host: Goat

Clonality: Polyclonal

Conjugate: This RFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-RFP Antibody

Purpose: RFP Polyclonal Antibody

Specificity: In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).

Cross-Reactivity (Details): Red Fluorescent Protein (dsRed), mCherry, mOrange, mPlum, mRFP, mStrawberry and tdTomato

Purification: epitope affinity purified

Immunogen: Purified recombinant RFP peptide produced in E. coli.

Isotype: IgG

Application Details

Application Notes: Western blot 1:500-1:2,000
Immunofluorescence 1:50-1:500
Immunohistochemistry (paraffin) 1:50-1:500
Immunohistochemistry (frozen) 1:50-1:500

Restrictions: For Research Use only

Handling

Handling Advice: The antibody solution should be gently mixed before use.

Storage: 4 °C/-20 °C

Storage Comment: For continuous use, store at 2-8°C for one-two days. For extended storage, store in -20°C freezer. Working dilution samples should be discarded if not used within 12 hours.

References for anti-RFP antibody (ABIN6254205)

Krishna, Choudhury, Keough, Seo, Ni, Kakaizada, Lee, Aabedi, Popova, Lipkin, Cao, Nava Gonzales, Sudharshan, Egladyous, Almeida, Zhang, Molinaro, Venkatesh, Daniel, Shamardani, Hyer, Chang, Findlay, Phillips, Nagarajan, Raleigh, Brang, Monje, Hervey-Jumpe: "Glioblastoma remodelling of human neural circuits decreases survival." in: Nature, Vol. 617, Issue 7961, pp. 599-607, (2023) (PubMed).

Drexler, Khatri, Sauvigny, Mohme, Maire, Ryba, Zghaibeh, Dührsen, Salviano-Silva, Lamszus, Westphal, Gempt, Wefers, Neumann, Bode, Hausmann, Huber, Bonn, Jütten, Delev, Weber, Harter, Onken, Vajkoczy, Capper, Wiestler, Weller, Snijder, Buck, Weiss, Keough: "Epigenetic neural glioblastoma enhances synaptic integration and predicts therapeutic vulnerability." in: bioRxiv : the preprint server for biology, (2023) (PubMed).

Semerci, Choi, Bajic, Thakkar, Encinas, Depreux, Segil, Groves, Maletic-Savatic: "Lunatic fringe-mediated Notch signaling regulates adult hippocampal neural stem cell maintenance." in: eLife, Vol. 6, (2018) (PubMed).

Tian, Peng, Murugan, Feng, Liu, Eyo, Zhou, Mogilevsky, Wang, Wu: "Chemokine CCL2-CCR2 Signaling Induces Neuronal Cell Death via STAT3 Activation and IL-1β Production after Status Epilepticus." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 33, pp. 7878-7892, (2017) (PubMed).

Target Details for RFP

Target: RFP (Red Fluorescent Protein (RFP))

Alternative Name: RFP