CDC25B antibody (pSer353)

Catalog No. ABIN6255250

The Rabbit Polyclonal anti-CDC25B antibody (ABIN6255250) specifically detects CDC25B in WB, ELISA, IHC, IF and ICC. The antibody is reactive with Human and Mouse samples.

Quick Overview for CDC25B antibody (pSer353) (ABIN6255250)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CDC25B Antibody

Binding Specificity: pSer353

Specificity: Phospho-CDC25B (Ser353) Antibody detects endogenous levels of CDC25B only when phosphorylated at Serine 353

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CDC25B around the phosphorylation site of Serine 353

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B

Background:

Description: Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.

Gene: CDC25B

Molecular Weight: 64kDa

Gene ID: 994

UniProt: P30305

Pathways: Cell Division Cycle, M Phase, Autophagy