ATG13 antibody (pSer355)

Catalog No. ABIN6255252

The Rabbit Polyclonal anti-ATG13 antibody is suitable to detect ATG13 in samples from Human, Mouse and Rat. It has been validated for WB and IHC.

Quick Overview for ATG13 antibody (pSer355) (ABIN6255252)

Target: ATG13 (Autophagy Related 13 (ATG13))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ATG13 Antibody

Binding Specificity: pSer355

Specificity: Phospho-Atg13 (Ser355) Antibody detects endogenous levels of Atg13.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Atg13 around the phosphorylation site of Ser355.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for ATG13

Target: ATG13 (Autophagy Related 13 (ATG13))

Alternative Name: Atg13

Background:

Description: Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.

Gene: ATG13

Molecular Weight: 72kDa

Gene ID: 9776

UniProt: O75143

Pathways: PI3K-Akt Signaling, Autophagy