EEF2K antibody (pSer366)

Catalog No. ABIN6255261

The Rabbit Polyclonal anti-EEF2K antibody is suitable to detect EEF2K in samples from Human and Mouse. It has been validated for WB and IHC.

Quick Overview for EEF2K antibody (pSer366) (ABIN6255261)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-EEF2K Antibody

Binding Specificity: pSer366

Specificity: Phospho-eEF2k (Ser366) Antibody detects endogenous levels of eEF2k.

Cross-Reactivity: Human, Mouse (Murine)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human eEF2k around the phosphorylation site of Ser366.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C.Stable for 12 months from date of receipt

Expiry Date: 12 months

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: eEF2k

Background:

Description: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Gene: EEF2K

Molecular Weight: 105kDa

Gene ID: 29904

UniProt: O00418

Pathways: AMPK Signaling