Caspase 1 antibody (pSer376)

Catalog No. ABIN6255274

This anti-Caspase 1 antibody is a Rabbit Polyclonal antibody detecting Caspase 1 in WB, ELISA, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for Caspase 1 antibody (pSer376) (ABIN6255274)

Target: Caspase 1 (CASP1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-Caspase 1 Antibody

Binding Specificity: pSer376

Specificity: Phospho-Caspase 1 (Ser376) Antibody detects endogenous levels of Caspase 1 only when phosphorylated at Ser376.

Predicted Reactivity: Pig,Bovine,Rabbit

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Caspase 1 around the phosphorylation site of Ser376.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: CASP1

Background:

Description: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590).

Gene: CASP1

Molecular Weight: 45 kDa

Gene ID: 834

UniProt: P29466

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome