JAK1 antibody (pTyr1022, pTyr1023)

Catalog No. ABIN6255762

The Rabbit Polyclonal anti-JAK1 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect JAK1 in samples from Human, Mouse and Rat. There are 4+ publications available.

Quick Overview for JAK1 antibody (pTyr1022, pTyr1023) (ABIN6255762)

Target: JAK1 (Janus Kinase 1 (JAK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-JAK1 Antibody

Binding Specificity: pTyr1022, pTyr1023

Specificity: Phospho-JAK1 (Tyr1022/Tyr1023) Antibody detects endogenous levels of JAK1 only when phosphorylated at Tyr1034/Tyr1035, which site historically referenced as Tyr1022/Tyr1023.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human JAK1 around the phosphorylation site of Tyr1022/Tyr1023.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:500, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-JAK1 antibody (ABIN6255762)

Fukasawa, Yoshizaki, Ebata, Yoshizaki-Ogawa, Asano, Enomoto, Miyagawa, Kazoe, Mawatari, Kitamori, Sato: "Single-cell-level protein analysis revealing the roles of autoantigen-reactive B lymphocytes in autoimmune disease and the murine model." in: eLife, Vol. 10, (2021) (PubMed).

Chen, Zhou, Liu, Huang, Liu, Kang, Chen, Guo, Zhu, Sun: "Combination of gemcitabine and erlotinib inhibits recurrent pancreatic cancer growth in mice via the JAK-STAT pathway." in: Oncology reports, Vol. 39, Issue 3, pp. 1081-1089, (2018) (PubMed).

Tu, Yu, Zhang, Shi, Xu, Hu, Gan, He, Dong, Han, Huang, Pan, Zhang: "A novel fluorinated triazole derivative suppresses macrophage activation and alleviates experimental colitis via a Twist1-dependent pathway." in: Biochemical pharmacology, Vol. 155, pp. 275-287, (2018) (PubMed).

Bai, Wang, Han, Xie, Ji, Yin, Chen, Wang, Jiang, Qi, Jiang: "BCL2L10 inhibits growth and metastasis of hepatocellular carcinoma both in vitro and in vivo." in: Molecular carcinogenesis, Vol. 56, Issue 3, pp. 1137-1149, (2017) (PubMed).

Target Details for JAK1

Target: JAK1 (Janus Kinase 1 (JAK1))

Alternative Name: JAK1

Background:

Description: Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway (PubMed:7615558). Kinase partner for the interleukin (IL)-2 receptor (PubMed:11909529).

Gene: JAK1

Molecular Weight: 130kDa

Gene ID: 3716

UniProt: P23458

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded Protein Response