CREB1 antibody (pSer133)

Catalog No. ABIN6256130

This Rabbit Polyclonal antibody specifically detects CREB1 in WB, IHC, ELISA, IF, IP and ICC. It exhibits reactivity toward Human, Mouse and Rat.It has been mentioned in 1 publication

Quick Overview for CREB1 antibody (pSer133) (ABIN6256130)

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CREB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CREB1 Antibody

Binding Specificity: pSer133

Specificity: Phospho-CREB (Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at Serine 133.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CREB around the phosphorylation site of Ser133.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:100-1:500, IP, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-CREB1 antibody (ABIN6256130)

Wang, Zhou, Guan, Yu, Wang: "β-Estradiol antagonizes the inhibitory effects of caffeine in BMMSCs via the ERβ-mediated cAMP-dependent PKA pathway." in: Toxicology, Vol. 394, pp. 1-10, (2018) (PubMed).

Target Details for CREB1

Target: CREB1 (cAMP Responsive Element Binding Protein 1 (CREB1))

Alternative Name: CREB1

Background:

Description: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells.

Gene: CREB1

Molecular Weight: 43kDa

Gene ID: 1385

UniProt: P16220

Pathways: TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation