CDC25C antibody (pSer216)

Catalog No. ABIN6256197

The Rabbit Polyclonal anti-CDC25C antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect CDC25C in samples from Human and Mouse.

Quick Overview for CDC25C antibody (pSer216) (ABIN6256197)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CDC25C Antibody

Binding Specificity: pSer216

Specificity: Phospho-CDC25C (Ser216) Antibody detects endogenous levels of CDC25C only when phosphorylated at Serine 216.

Predicted Reactivity: Horse,Dog,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CDC25C around the phosphorylation site of Ser216.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: CDC25C

Background:

Description: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Gene: CDC25C

Molecular Weight: 60kDa

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase