NCF1 antibody (pSer359)

Catalog No. ABIN6256289

This Rabbit Polyclonal antibody specifically detects NCF1 in WB, ELISA, IHC, IF and ICC. It exhibits reactivity toward Human and Rat.

Quick Overview for NCF1 antibody (pSer359) (ABIN6256289)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-NCF1 Antibody

Binding Specificity: pSer359

Specificity: Phospho-NCF1/p47-phox (Ser359) Antibody detects endogenous levels of NCF1/p47-phox only when phosphorylated at Serine 359.

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human NCF1/p47-phox around the phosphorylation site of Ser359.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Alternative Name: NCF1

Background:

Description: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Gene: NCF1

Molecular Weight: 45kDa

Gene ID: 653361

UniProt: P14598

Pathways: PI3K-Akt Signaling