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Vimentin antibody (pSer56)

This anti-Vimentin antibody is a Rabbit Polyclonal antibody detecting Vimentin in WB, IHC, ELISA, IF and ICC. Suitable for Human.
Catalog No. ABIN6256375

Quick Overview for Vimentin antibody (pSer56) (ABIN6256375)

Target

See all Vimentin (VIM) Antibodies
Vimentin (VIM)

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This Vimentin antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Binding Specificity

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    pSer56

    Specificity

    Phospho-Vimentin (Ser56) Antibody detects endogenous levels of Vimentin only when phosphorylated at Serine 56.

    Predicted Reactivity

    Pig,Bovine,Sheep,Dog

    Purification

    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

    Immunogen

    A synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56.

    Isotype

    IgG
  • Application Notes

    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    -20 °C

    Storage Comment

    Store at -20 °C. Stable for 12 months from date of receipt.

    Expiry Date

    12 months
  • Target

    Vimentin (VIM)

    Alternative Name

    VIM

    Background

    Description: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

    Gene: VIM

    Molecular Weight

    54kDa

    Gene ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Cascade in Apoptosis
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