Vimentin antibody (pSer56)
Quick Overview for Vimentin antibody (pSer56) (ABIN6256375)
Target
See all Vimentin (VIM) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pSer56
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Specificity
- Phospho-Vimentin (Ser56) Antibody detects endogenous levels of Vimentin only when phosphorylated at Serine 56.
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Predicted Reactivity
- Pig,Bovine,Sheep,Dog
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Purification
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
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Immunogen
- A synthesized peptide derived from human Vimentin around the phosphorylation site of Ser56.
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Isotype
- IgG
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Application Notes
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C. Stable for 12 months from date of receipt.
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Expiry Date
- 12 months
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- Vimentin (VIM)
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Alternative Name
- VIM
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Background
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Description: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Gene: VIM
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Molecular Weight
- 54kDa
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Gene ID
- 7431
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UniProt
- P08670
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Pathways
- Caspase Cascade in Apoptosis
Target
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