CaMKIIalpha/delta antibody (pThr286)

Catalog No. ABIN6256533

The Rabbit Polyclonal anti-CaMKIIalpha/delta antibody is suitable to detect CaMKIIalpha/delta in samples from Human, Mouse and Rat. It has been validated for IF, WB, IHC and ICC.

Quick Overview for CaMKIIalpha/delta antibody (pThr286) (ABIN6256533)

Target: CaMKIIalpha/delta

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CaMKIIalpha/delta antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-CaMKIIalpha/delta Antibody

Binding Specificity: pThr286

Purpose: Phospho-CaMKII alpha/delta (Thr286) Antibody

Specificity: Phospho-CaMK2 (Thr286) Antibody detects endogenous levels of CaMK2 only when phosphorylated at Threonine 286

Predicted Reactivity: Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human CaMK2 alpha/delta around the phosphorylation site of Thr286.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CaMKIIalpha/delta

Target: CaMKIIalpha/delta

Alternative Name: CaMKII alpha/delta

Background:

Description: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity (By similarity). Phosphorylates transcription factor FOXO3 on 'Ser-298' (PubMed:23805378). Activates FOXO3 transcriptional activity (By similarity).

Gene: CAMK2A

Molecular Weight: 50kDa

Gene ID: 815

UniProt: Q9UQM7