CaMKIIalpha/delta antibody (pThr286)

Catalog No. ABIN7225194

The Rabbit Polyclonal anti-CaMKIIalpha/delta antibody (ABIN7225194) specifically detects CaMKIIalpha/delta in IF, WB, IHC and ELISA. The antibody is reactive with Human, Mouse, Rat and Pig samples.

Quick Overview for CaMKIIalpha/delta antibody (pThr286) (ABIN7225194)

Target: CaMKIIalpha/delta

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CaMKIIalpha/delta antibody is un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-CaMKIIalpha/delta Antibody

Binding Specificity: pThr286

Purpose: Rabbit Anti-CaMKIIα/δ (phospho Thr286) Polyclonal Antibody

Specificity: Phospho-CaMKIIα/δ (T286) Polyclonal Antibody detects endogenous levels of CaMKIIα/δ protein only when phosphorylated at T286.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CaMKIIalpha/delta Phospho-Thr286

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CaMKIIalpha/delta

Target: CaMKIIalpha/delta

Background: CAMK2A, CAMKA, KIAA0968, Calcium/calmodulin-dependent protein kinase type II subunit alpha, CaM kinase II subunit alpha, CaMK-II subunit alpha, CAMK2D, CAMKD, Calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase IIThe product of CAMK2A (calcium/calmodulin dependent protein kinase II alpha) belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for CAMK2A.

Molecular Weight: 54kD

Gene ID: 815, 817

UniProt: Q9UQM7, Q13557