Cyclin E1 antibody (pThr395)

Catalog No. ABIN6256557

The Rabbit Polyclonal anti-Cyclin E1 antibody has been validated for WB, IHC, ELISA, IF and ICC. It is suitable to detect Cyclin E1 in samples from Human.

Quick Overview for Cyclin E1 antibody (pThr395) (ABIN6256557)

Target: Cyclin E1 (CCNE1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Cyclin E1 Antibody

Binding Specificity: pThr395

Specificity: Phospho-Cyclin E1 (Thr395) Antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Threonine 395.

Predicted Reactivity: Bovine,Horse,Sheep

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human Cyclin E1 around the phosphorylation site of Thr395.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Cyclin E1

Target: Cyclin E1 (CCNE1)

Alternative Name: CCNE1

Background:

Description: Essential for the control of the cell cycle at the G1/S (start) transition.

Gene: CCNE1

Molecular Weight: 48kDa

Gene ID: 898

UniProt: P24864

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases