E2F1 antibody (pThr433)

Catalog No. ABIN6256563

The Rabbit Polyclonal anti-E2F1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect E2F1 in samples from Human and Mouse.

Quick Overview for E2F1 antibody (pThr433) (ABIN6256563)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-E2F1 Antibody

Binding Specificity: pThr433

Specificity: Phospho-E2F1 (Thr433) Antibody detects endogenous levels of E2F1 only when phosphorylated at Threonine 433.

Predicted Reactivity: Pig,Bovine,Horse,Rabbit,Chicken

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogen: A synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background:

Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Positively regulates transcription of RRP1B (PubMed:20040599).

Gene: E2F1

Molecular Weight: 47kDa

Gene ID: 1869

UniProt: Q01094

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy