Crossover junction endonuclease EME1 (EME1) (Internal Region) antibody

Catalog No. ABIN6256869

The Rabbit Polyclonal anti- antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect in samples from Human.

Quick Overview for Crossover junction endonuclease EME1 (EME1) (Internal Region) antibody (ABIN6256869)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details

Binding Specificity: Internal Region

Specificity: EME1 Antibody detects endogenous levels of total EME1.

Predicted Reactivity: Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EME1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1

Background:

Description: Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

Gene: EME1

Molecular Weight: 65 kDa

Gene ID: 146956

UniProt: Q96AY2