Crossover junction endonuclease EME1 (EME1) (AA 250-330) antibody

Catalog No. ABIN7219782

The Rabbit Polyclonal anti-Crossover junction endonuclease EME1 antibody is suitable to detect Crossover junction endonuclease EME1 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for Crossover junction endonuclease EME1 (EME1) (AA 250-330) antibody (ABIN7219782)

Target: Crossover junction endonuclease EME1 (EME1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Product Details

Binding Specificity: AA 250-330

Purpose: Rabbit Anti-Eme1 Polyclonal Antibody

Specificity: Eme1 Polyclonal Antibody detects endogenous levels of Eme1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Eme1 at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: Eme1

Background: EME1, MMS4, Crossover junction endonuclease EME1, MMS4 homolog, hMMS4EME1 encodes essential meiotic structure-specific endonuclease 1 that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.

Molecular Weight: 65kD

Gene ID: 146956

UniProt: Q96AY2