EIF3K antibody (N-Term)

Catalog No. ABIN6257176

This anti-EIF3K antibody is a Rabbit Polyclonal antibody detecting EIF3K in WB, ELISA, ICC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for EIF3K antibody (N-Term) (ABIN6257176)

Target: EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-EIF3K Antibody

Binding Specificity: N-Term

Specificity: EIF3K Antibody detects endogenous levels of total EIF3K.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human EIF3K, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for EIF3K

Target: EIF3K (Eukaryotic Translation Initiation Factor 3 Subunit K (EIF3K))

Alternative Name: EIF3K

Background:

Description: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Gene: EIF3K

Molecular Weight: 30 kDa

Gene ID: 27335

UniProt: Q9UBQ5

Pathways: Ribonucleoprotein Complex Subunit Organization