MAPKSP1 antibody (Internal Region)

Catalog No. ABIN6257364

The Rabbit Polyclonal anti-MAPKSP1 antibody is suitable to detect MAPKSP1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and ICC.

Quick Overview for MAPKSP1 antibody (Internal Region) (ABIN6257364)

Target: MAPKSP1 (Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPKSP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MAPKSP1 Antibody

Binding Specificity: Internal Region

Specificity: LAMTOR3 Antibody detects endogenous levels of total LAMTOR3.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LAMTOR3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for MAPKSP1

Target: MAPKSP1 (Mitogen-Activated Protein Kinase Kinase 1 Interacting Protein 1 (MAPKSP1))

Alternative Name: LAMTOR3

Background:

Description: As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.

Gene: LAMTOR3

Gene ID: 8649

UniProt: Q9UHA4

Pathways: PI3K-Akt Signaling