USP37 antibody (Internal Region)

Catalog No. ABIN6257545

Product Details anti-USP37 Antibody

Target: USP37 (Ubiquitin Specific Peptidase 37 (USP37))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP37 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: USP37 Antibody detects endogenous levels of total USP37.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human USP37, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for USP37

Target: USP37 (Ubiquitin Specific Peptidase 37 (USP37))

Alternative Name: USP37 (USP37 Products)

Synonyms: 4932415L06Rik antibody, C330008N13Rik antibody, wu:fi15b04 antibody, wu:fi38d03 antibody, zgc:152882 antibody, zgc:153999 antibody, ubiquitin specific peptidase 37 antibody, USP37 antibody, usp37 antibody, Usp37 antibody

Background:

Description: Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (PubMed:21596315). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1 (PubMed:27296872).

Gene: USP37

Molecular Weight: ?120 kDa

Gene ID: 57695

UniProt: Q86T82