CD55 antibody (Internal Region)

Catalog No. ABIN6258004

The Rabbit Polyclonal anti-CD55 antibody (ABIN6258004) specifically detects CD55 in WB, IHC, IF and ICC. The antibody is reactive with Human and Rat samples.

Quick Overview for CD55 antibody (Internal Region) (ABIN6258004)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-CD55 Antibody

Binding Specificity: Internal Region

Purpose: CD55 Antibody

Specificity: CD55 Antibody detects endogenous levels of total CD55.

Predicted Reactivity: Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CD55, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background:

Description: This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829).

Gene: CD55

Molecular Weight: 41 kDa

Gene ID: 1604

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity