DOT1L antibody (C-Term)

Catalog No. ABIN6258125

This Rabbit Polyclonal antibody specifically detects DOT1L in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for DOT1L antibody (C-Term) (ABIN6258125)

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOT1L antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-DOT1L Antibody

Binding Specificity: C-Term

Specificity: DOT1L Antibody detects endogenous levels of total DOT1L.

Predicted Reactivity: Bovine,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DOT1L, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for DOT1L

Target: DOT1L (DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae) (DOT1L))

Alternative Name: DOT1L

Background:

Description: Histone methyltransferase. Methylates 'Lys-79' of histone H3. Nucleosomes are preferred as substrate compared to free histones. Binds to DNA.

Gene: DOT1L

Molecular Weight: 185 kDa.

Gene ID: 84444

UniProt: Q8TEK3