SIRT1 antibody (AA 577-590)

Catalog No. ABIN625834

This Goat Polyclonal antibody specifically detects SIRT1 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for SIRT1 antibody (AA 577-590) (ABIN625834)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SIRT1 Antibody

Binding Specificity: AA 577-590

Purpose: Sirtuin 1 / SIRT1 (aa577-590)

Sequence: EKPQEVQTSR NVES

Specificity: This antibody is expected to recognize both reported isoforms (NP_036370.2, NP_001135970.1).

Cross-Reactivity: Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2, NP_001135970.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 120 kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7 kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Ac

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Alternative Name: SIRT1

Background: SIRT1, sirtuin 1, SIR2L1, NAD-dependent deacetylase sirtuin-1, OTTHUMP00000198111, OTTHUMP00000198112, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, sir2-like 1, sirtuin type 1

Gene ID: 23411, 93759

NCBI Accession: NP_036370, NP_001135970

Pathways: MAPK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling