BHLHE41 antibody (N-Term)

Catalog No. ABIN6258612

The Rabbit Polyclonal anti-BHLHE41 antibody is suitable to detect BHLHE41 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and ICC.

Quick Overview for BHLHE41 antibody (N-Term) (ABIN6258612)

Target: BHLHE41 (Basic Helix-Loop-Helix Family, Member E41 (BHLHE41))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BHLHE41 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-BHLHE41 Antibody

Binding Specificity: N-Term

Specificity: BHLHB3 Antibody detects endogenous levels of total BHLHB3.

Predicted Reactivity: Pig,Bovine,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human BHLHB3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for BHLHE41

Target: BHLHE41 (Basic Helix-Loop-Helix Family, Member E41 (BHLHE41))

Alternative Name: BHLHE41

Background:

Description: Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity.

Gene: BHLHE41

Molecular Weight: 50 kDa

Gene ID: 79365

UniProt: Q9C0J9

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development