POLR3C antibody (Internal Region)

Catalog No. ABIN6259155

The Rabbit Polyclonal anti-POLR3C antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect POLR3C in samples from Human.

Quick Overview for POLR3C antibody (Internal Region) (ABIN6259155)

Target: POLR3C (Polymerase (RNA) III (DNA Directed) Polypeptide C (62kD) (POLR3C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR3C Antibody

Binding Specificity: Internal Region

Specificity: RPC3 Antibody detects endogenous levels of total RPC3.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human RPC3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for POLR3C

Target: POLR3C (Polymerase (RNA) III (DNA Directed) Polypeptide C (62kD) (POLR3C))

Alternative Name: POLR3C

Background:

Description: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. Preferentially binds single-stranded DNA (ssDNA) in a sequence-independent manner (PubMed:21358628).

Gene: POLR3C

Molecular Weight: 60 kDa

Gene ID: 10623

UniProt: Q9BUI4