Cytochrome b antibody (N-Term)

Catalog No. ABIN6259564

The Rabbit Polyclonal anti-Cytochrome b antibody (ABIN6259564) specifically detects Cytochrome b in ELISA, WB, IHC, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Cytochrome b antibody (N-Term) (ABIN6259564)

Target: Cytochrome b (MT-CYB) (Mitochondrially Encoded Cytochrome B (MT-CYB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome b antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Cytochrome b Antibody

Binding Specificity: N-Term

Specificity: MT-CYB Antibody detects endogenous levels of total MT-CYB.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human MT-CYB, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Expiry Date: 12 months

Target Details for Cytochrome b

Target: Cytochrome b (MT-CYB) (Mitochondrially Encoded Cytochrome B (MT-CYB))

Alternative Name: MT-CYB

Background:

Description: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.

Gene: MT-CYB

Molecular Weight: 43 kDa

Gene ID: 4519

UniProt: P00156

Pathways: Proton Transport