SIX Homeobox 1 antibody (Internal Region)

Catalog No. ABIN6259572

The Rabbit Polyclonal anti-SIX Homeobox 1 antibody has been validated for WB, ELISA, IF and ICC. It is suitable to detect SIX Homeobox 1 in samples from Human and Mouse.

Quick Overview for SIX Homeobox 1 antibody (Internal Region) (ABIN6259572)

Target: SIX Homeobox 1 (SIX1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIX Homeobox 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SIX Homeobox 1 Antibody

Binding Specificity: Internal Region

Specificity: SIX1 Antibody detects endogenous levels of total SIX1.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SIX1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SIX Homeobox 1

Target: SIX Homeobox 1 (SIX1)

Alternative Name: SIX1

Background:

Description: Transcription factor that is involved in the regulation of cell proliferation, apoptosis and embryonic development. Plays an important role in the development of several organs, including kidney, muscle and inner ear. Depending on context, functions as transcriptional repressor or activator. Lacks an activation domain, and requires interaction with EYA family members for transcription activation. Mediates nuclear translocation of EYA1 and EYA2. Binds the 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the MYOG promoter. Regulates the expression of numerous genes, including MYC, CCND1 and EZR. Acts as activator of the IGFBP5 promoter, probably coactivated by EYA2. Repression of precursor cell proliferation in myoblasts is switched to activation through recruitment of EYA3 to the SIX1-DACH1 complex. During myogenesis, seems to act together with EYA2 and DACH2 (By similarity). Regulates the expression of CCNA1.

Gene: SIX1

Molecular Weight: 33 kDa

Gene ID: 6495

UniProt: Q15475

Pathways: Sensory Perception of Sound, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development