alpha Actinin antibody (N-Term)

Catalog No. ABIN6259752

The Rabbit Polyclonal anti-alpha Actinin antibody is suitable to detect alpha Actinin in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for alpha Actinin antibody (N-Term) (ABIN6259752)

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Actinin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-alpha Actinin Antibody

Binding Specificity: N-Term

Purpose: alpha-Actinin Antibody

Specificity: Alpha-Actinin Antibody detects endogenous levels of total alpha-Actinin.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human alpha-Actinin, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for alpha Actinin

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Alternative Name: alpha-Actinin

Background:

Description: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

Gene: ACTN1

Molecular Weight: 103kDa

Gene ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization, Negative Regulation of Transporter Activity