PRKAG2 antibody (N-Term)

Catalog No. ABIN6259920

Product Details anti-PRKAG2 Antibody

Target: PRKAG2 (Protein Kinase, AMP-Activated, gamma 2 Non-Catalytic Subunit (PRKAG2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: AMPKgamma2 Antibody detects endogenous levels of total AMPKgamma2.

Predicted Reactivity: Pig,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human AMPKgamma2, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:1000, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for PRKAG2

Target: PRKAG2 (Protein Kinase, AMP-Activated, gamma 2 Non-Catalytic Subunit (PRKAG2))

Alternative Name: PRKAG2 (PRKAG2 Products)

Synonyms: MGC82938 antibody, PRKAG2 antibody, si:ch211-230l11.1 antibody, si:dkey-217f4.2 antibody, AAKG antibody, AAKG2 antibody, CMH6 antibody, H91620p antibody, WPWS antibody, 2410051C13Rik antibody, AI854673 antibody, protein kinase AMP-activated non-catalytic subunit gamma 2 antibody, protein kinase, AMP-activated, gamma 2 non-catalytic subunit L homeolog antibody, 5'-AMP-activated protein kinase subunit gamma-2 antibody, protein kinase, AMP-activated, gamma 2 non-catalytic subunit b antibody, protein kinase, AMP-activated, gamma 2 non-catalytic subunit antibody, Prkag2 antibody, PRKAG2 antibody, prkag2.L antibody, LOC504219 antibody, prkag2b antibody, prkag2 antibody

Background:

Description: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.

Gene: PRKAG2

Molecular Weight: 65kDa

Gene ID: 51422

UniProt: Q9UGJ0

Pathways: AMPK Signaling, Cellular Glucan Metabolic Process, Ribonucleoside Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Warburg Effect