APLF antibody (Internal Region)

Catalog No. ABIN6259979

This Rabbit Polyclonal antibody specifically detects APLF in ELISA, WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for APLF antibody (Internal Region) (ABIN6259979)

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APLF antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-APLF Antibody

Binding Specificity: Internal Region

Specificity: APLF Antibody detects endogenous levels of total APLF.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human APLF, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000, IHC: 1:50-1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for APLF

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Alternative Name: APLF

Background:

Description: Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.

Gene: APLF

Molecular Weight: 57kDa

Gene ID: 200558

UniProt: Q8IW19

Pathways: DNA Damage Repair