APLF antibody

Catalog No. ABIN7220244

The Rabbit Polyclonal anti-APLF antibody (ABIN7220244) specifically detects APLF in ELISA, IHC, WB and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for APLF antibody (ABIN7220244)

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APLF antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-APLF Antibody

Purpose: Rabbit Anti-APLF Polyclonal Antibody

Specificity: APLF Polyclonal Antibody detects endogenous levels of APLF protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human APLF around the non-phosphorylation site of S116

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for APLF

Target: APLF (Aprataxin and PNKP Like Factor (APLF))

Alternative Name: APLF

Background: APLF, C2orf13, PALF, XIP1, Aprataxin and PNK-like factor, Apurinic-apyrimidinic endonuclease APLF, PNK and APTX-like FHA domain-containing protein, XRCC1-interacting protein 1C2ORF13 is a component of the cellular response to chromosomal DNA single- and double-strand breaks (Iles et al. 2007 [PubMed 17353262]).

Molecular Weight: 57kD

Gene ID: 200558

UniProt: Q8IW19

Pathways: DNA Damage Repair