Cyclin F antibody (C-Term)

Catalog No. ABIN6261153

The Rabbit Polyclonal anti-Cyclin F antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect Cyclin F in samples from Human.

Quick Overview for Cyclin F antibody (C-Term) (ABIN6261153)

Target: Cyclin F (CCNF)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Cyclin F Antibody

Binding Specificity: C-Term

Specificity: Cyclin F Antibody detects endogenous levels of total Cyclin F.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Cyclin F, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Cyclin F

Target: Cyclin F (CCNF)

Alternative Name: CCNF

Background:

Description: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication.

Gene: CCNF

Molecular Weight: 87 kDa

Gene ID: 899

UniProt: P41002