Dihydrofolate Reductase antibody (N-Term)

Catalog No. ABIN6261285

The Rabbit Polyclonal anti-Dihydrofolate Reductase antibody is suitable to detect Dihydrofolate Reductase in samples from Human, Mouse, Rat and Monkey. It has been validated for WB, IHC, IF and ICC.

Quick Overview for Dihydrofolate Reductase antibody (N-Term) (ABIN6261285)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Dihydrofolate Reductase Antibody

Binding Specificity: N-Term

Purpose: DHFR Antibody

Specificity: DHFR Antibody detects endogenous levels of total DHFR

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DHFR, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background:

Description: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Gene: DHFR

Molecular Weight: 21kDa

Gene ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases