DIAPH3 antibody (Internal Region)

Catalog No. ABIN6261297

The Rabbit Polyclonal anti-DIAPH3 antibody has been validated for WB, IF, IHC and ICC. It is suitable to detect DIAPH3 in samples from Human, Rat and Mouse.

Quick Overview for DIAPH3 antibody (Internal Region) (ABIN6261297)

Target: DIAPH3 (Diaphanous Homolog 3 (Drosophila) (DIAPH3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIAPH3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-DIAPH3 Antibody

Binding Specificity: Internal Region

Specificity: DIAPH3 Antibody detects endogenous levels of total DIAPH3.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human DIAPH3, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for DIAPH3

Target: DIAPH3 (Diaphanous Homolog 3 (Drosophila) (DIAPH3))

Alternative Name: DIAPH3

Background:

Description: Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).

Gene: DIAPH3

Molecular Weight: 125-137 kDa

Gene ID: 81624

UniProt: Q9NSV4