EEF2K antibody (Internal Region)

Catalog No. ABIN6261444

The Rabbit Polyclonal anti-EEF2K antibody has been validated for WB, ELISA, IHC, IF and ICC. It is suitable to detect EEF2K in samples from Human, Rat and Mouse. There is 1 publication available.

Quick Overview for EEF2K antibody (Internal Region) (ABIN6261444)

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEF2K antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-EEF2K Antibody

Binding Specificity: Internal Region

Specificity: EEF2K Antibody detects endogenous levels of total eEF2K.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human eEF2K, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-EEF2K antibody (ABIN6261444)

Deng, Luo, Lai, Song, Peng, Wei: "Myostatin inhibits eEF2K-eEF2 by regulating AMPK to suppress protein synthesis." in: Biochemical and biophysical research communications, Vol. 494, Issue 1-2, pp. 278-284, (2017) (PubMed).

Target Details for EEF2K

Target: EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))

Alternative Name: EEF2K

Background:

Description: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.

Gene: EEF2K

Molecular Weight: 82kDa

Gene ID: 29904

UniProt: O00418

Pathways: AMPK Signaling