ERCC1 antibody (Internal Region)

Catalog No. ABIN6261588

The Rabbit Polyclonal anti-ERCC1 antibody is suitable to detect ERCC1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and ICC.

Quick Overview for ERCC1 antibody (Internal Region) (ABIN6261588)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ERCC1 Antibody

Binding Specificity: Internal Region

Specificity: ERCC1 Antibody detects endogenous levels of total ERCC1.

Predicted Reactivity: Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human ERCC1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Alternative Name: ERCC1

Background:

Description: Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.

Gene: ERCC1

Molecular Weight: 33kDa

Gene ID: 2067

UniProt: P07992

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response