Fibronectin antibody (C-Term)

Catalog No. ABIN6261746

This Rabbit Polyclonal antibody specifically detects Fibronectin in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat. It has been mentioned in 2+ publications

Quick Overview for Fibronectin antibody (C-Term) (ABIN6261746)

Target: Fibronectin

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Fibronectin Antibody

Binding Specificity: C-Term

Purpose: Fibronectin Antibody

Specificity: Fibronectin Antibody detects endogenous levels of total Fibronectin.

Predicted Reactivity: Pig,Bovine,Sheep,Rabbit

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Fibronectin, corresponding to a region within C-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

References for anti-Fibronectin antibody (ABIN6261746)

Yang, Zhou, Zhu, Fan, Liu, Duan, Tang, Shou, Zuo: "miR-200b-containing microvesicles attenuate experimental colitis associated intestinal fibrosis by inhibiting epithelial-mesenchymal transition." in: Journal of gastroenterology and hepatology, Vol. 32, Issue 12, pp. 1966-1974, (2018) (PubMed).

Xu, Sun, Fang, Song, Yan, Chen, Jiang, Fei, Zhao, Leboeuf, Li, Wang, Janin, Wang, Zhao: "JAM-A overexpression is related to disease progression in diffuse large B-cell lymphoma and downregulated by lenalidomide." in: Scientific reports, Vol. 7, Issue 1, pp. 7433, (2018) (PubMed).

Target Details for Fibronectin

Target: Fibronectin

Background:

Description: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Gene: FN1

Molecular Weight: 263 kDa

Gene ID: 2335

UniProt: P02751