Granzyme B/H (N-Term) antibody

Catalog No. ABIN6262072

The Rabbit Polyclonal anti-Granzyme B/H antibody (ABIN6262072) specifically detects Granzyme B/H in IHC, WB, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Granzyme B/H (N-Term) antibody (ABIN6262072)

Target: Granzyme B/H

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details

Binding Specificity: N-Term

Purpose: Granzyme B/H Antibody

Specificity: Granzyme B antibody detects endogenous levels of total Granzyme B

Predicted Reactivity: Pig,Bovine,Horse,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human Granzyme B/H, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details

Target: Granzyme B/H

Background:

Description: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Gene: GZMB

Molecular Weight: 28kDa

Gene ID: 3002

UniProt: P10144