HSPB11 antibody (N-Term)

Catalog No. ABIN6262479

The Rabbit Polyclonal anti-HSPB11 antibody is suitable to detect HSPB11 in samples from Human, Mouse and Rat. It has been validated for WB, IF, IHC and ICC.

Quick Overview for HSPB11 antibody (N-Term) (ABIN6262479)

Target: HSPB11 (Heat Shock Protein Family B (Small), Member 11 (HSPB11))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPB11 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-HSPB11 Antibody

Binding Specificity: N-Term

Purpose: IFT25 Antibody

Specificity: IFT25 Antibody detects endogenous levels of total IFT25.

Predicted Reactivity: Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human IFT25, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for HSPB11

Target: HSPB11 (Heat Shock Protein Family B (Small), Member 11 (HSPB11))

Alternative Name: IFT25

Background:

Description: Component of the IFT complex B required for sonic hedgehog/SHH signaling. May mediate transport of SHH components: required for the export of SMO and PTCH1 receptors out of the cilium and the accumulation of GLI2 at the ciliary tip in response to activation of the SHH pathway, suggesting it is involved in the dynamic transport of SHH signaling molecules within the cilium. Not required for ciliary assembly (By similarity).

Gene: HSPB11

Molecular Weight: 25 kDa

Gene ID: 51668

UniProt: Q9Y547