LAPTM4B antibody (Internal Region)

Catalog No. ABIN6262909

Product Details anti-LAPTM4B Antibody

Target: LAPTM4B (Lysosomal Protein Transmembrane 4 beta (LAPTM4B))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAPTM4B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: LAPTM4B Antibody detects endogenous levels of total LAPTM4B.

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LAPTM4B, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for LAPTM4B

Target: LAPTM4B (Lysosomal Protein Transmembrane 4 beta (LAPTM4B))

Alternative Name: LAPTM4B (LAPTM4B Products)

Synonyms: LAPTM4beta antibody, LC27 antibody, C330023P13Rik antibody, lysosomal protein transmembrane 4 beta antibody, lysosomal-associated protein transmembrane 4B antibody, LAPTM4B antibody, Laptm4b antibody

Background:

Description: Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656).

Gene: LAPTM4B

Molecular Weight: 24 kDa

Gene ID: 55353

UniProt: Q86VI4